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Covalent glycated proteins accumulate in several tissues during aging and/or diabetic complications. These proteins can accelerate amyloid-beta (Aβ)-like protein deposition and Alzheimer’s disease (AD) progression and are known as advanced glycation end products (AGEs). Glycated proteins may be deposited in various tissues at sites with relatively low metabolism as in the central nervous system. In this study, the release of Aβ-like immunoreactivity (ALI) from bovine serum albumin (BSA)-AGEs conjugates was measured using an ELISA kit (i.e., the human Aβ(1-42)-specific ALICE kit). The results demonstrated that the addition of polysorbate 80, a surfactant, was the only factor affecting the release of ALI. The release of ALI was biphasic, with the first phase occurring within 1 h and the second phase occurring within 72 h. This release was inhibited by the addition of 5 mM sodium azide, a reactive oxygen species (ROS) scavenger. Furthermore, the inhibition was overcome by the addition of 100 µM ML-7, a GSK-3 inhibitor. The results suggest that the release of ALI depends on the presence of intact glycated proteins.Hydromorphone for relief of moderate to severe cancer pain.
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Mitral valve prostheses are commonly determined using two-dimensional and three-dimensional (3D) transesophageal echocardiography (T
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